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Dear Cherylclaire [Scientist's, not medic's opinions] come from some work I was involved in with a GP

Depressive-like symptoms are exacerbated by activation of P2X 7 R in glial cells

After Physical Activity or Illness If you've been physically active or recovering from illness, you may benefit from more frequent sessionsperhaps once every 2-4 weeks

After washing with PBS (3 5 min), sections were incubated with species-matched Alexa Fluor-conjugated secondary antibodies (1:500, Invitrogen) for 2 h at RT protected from light